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Analyse av Oversettelse Innvielse Under stressbetingelser ved Polysome Profilering
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Analyse av Oversettelse Innvielse Under stressbetingelser ved Polysome Profilering

Article DOI: 10.3791/51164 • 10:59 min
May 19th, 2014 •
*1,2, *1,2, 1,2
1Department of Molecular Biology, Medical Biochemistry, and Pathology, Faculty of Medicine, Laval University, 2CHU de Quebec Research Center
* These authors contributed equally

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May 19th, 2014

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Her beskriver vi en fremgangsmåte for å analysere endringer i initiering av mRNA oversettelse av eukaryote celler som svar på stressbetingelser. Denne metoden er basert på hastighetsseparasjon på sakkarosegradienter av sette ribosomer fra ikke-sette ribosomer.

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