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Proteomic Profiling of Macrophages by 2D Electrophoresis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Proteomic Profiling of Macrophages by 2D Electrophoresis

Proteomic Profiling of Macrophages by 2D Electrophoresis

DOI:
10.3791/52219-v

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07:53 min

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November 04, 2014

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, , , , , ,
1Inserm UMR744,University Lille Nord de France

Chapters

  • 00:05Title
  • 01:40Extraction of M1 (Pro-inflammatory) and M2 (Anti-inflammatory) Macrophage Proteins for 2D Electrophoresis
  • 03:55Isoelectrofocusing and Second Dimensional Electrophoresis
  • 05:29Image Acquisition and Bioinformatic Analysis
  • 06:25Results: Bioinformatic Analysis of M1 and M2 2D Protein Patterns
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Macrophages are the key cells involved in host pathogenicity. Macrophages display phenotypic and functional diversity that can be analysed and detected by proteomic analysis. This article describes how to perform 2D electrophoresis of primary cultures of human macrophages differentiated into M1 or M2 phenotype.

Tags

Keywords: Proteomic Profiling2D ElectrophoresisMacrophagesM1 PhenotypeM2 PhenotypeDIGEIsoelectric FocusingProtein ExpressionHost PathogenicityMicrobial Toxins

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