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JoVE Journal
Immunology and Infection

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Proteomic Profiling of Macrophages by 2D Electrophoresis
 

Proteomic Profiling of Macrophages by 2D Electrophoresis

Article DOI: 10.3791/52219
November 4th, 2014

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Summary November 4th, 2014

Macrophages are the key cells involved in host pathogenicity. Macrophages display phenotypic and functional diversity that can be analysed and detected by proteomic analysis. This article describes how to perform 2D electrophoresis of primary cultures of human macrophages differentiated into M1 or M2 phenotype.

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