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Proteomic Profiling of Macrophages by 2D Electrophoresis
Proteomic Profiling of Macrophages by 2D Electrophoresis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Proteomic Profiling of Macrophages by 2D Electrophoresis

Proteomic Profiling of Macrophages by 2D Electrophoresis

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07:53 min

November 04, 2014

DOI:

07:53 min
November 04, 2014

12660 Views
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Summary

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Macrophages are the key cells involved in host pathogenicity. Macrophages display phenotypic and functional diversity that can be analysed and detected by proteomic analysis. This article describes how to perform 2D electrophoresis of primary cultures of human macrophages differentiated into M1 or M2 phenotype.

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