12,688 Views
•
07:53 min
•
November 04, 2014
DOI:
Summary
Automatically generated
Macrophages are the key cells involved in host pathogenicity. Macrophages display phenotypic and functional diversity that can be analysed and detected by proteomic analysis. This article describes how to perform 2D electrophoresis of primary cultures of human macrophages differentiated into M1 or M2 phenotype.
Related Videos
12:34
Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
Related Videos
13406 Views
14:43
Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
Related Videos
15214 Views
10:15
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
Related Videos
7960 Views
11:25
Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
Related Videos
8810 Views
10:51
Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
Related Videos
16070 Views
10:18
From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
Related Videos
13710 Views
07:45
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
Related Videos
35468 Views
12:03
Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates
Related Videos
131188 Views
07:46
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
Related Videos
25213 Views
05:37
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Related Videos
6672 Views
Read Article
Cite this Article
Bouvet, M., Turkieh, A., Acosta-Martin, A. E., Chwastyniak, M., Beseme, O., Amouyel, P., Pinet, F. Proteomic Profiling of Macrophages by 2D Electrophoresis. J. Vis. Exp. (93), e52219, doi:10.3791/52219 (2014).
Copy