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JoVE Journal
Immunology and Infection

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A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide
 

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

Article DOI: 10.3791/51470
December 15th, 2014

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Summary December 15th, 2014

We describe a method for inducing neutrophilic pulmonary inflammation by challenge to aerosolized lipopolysaccharide by nebulization, to model acute lung injury. In addition, basic surgical techniques for lung isolation, tracheal intubation and bronchoalveolar lavage are also described.

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