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A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

English

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11,541 Views

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08:33 min

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December 15, 2014

DOI:

10.3791/51470-v

DOI:
10.3791/51470-v

•

08:33 min
December 15, 2014

•

1 Views
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1Department of Medicine, Solna and CMM, Respiratory Medicine Unit,Karolinska Institutet, 2Safety Science, Global Regulator Affairs & Patient Safety,AstraZeneca Global Medicines Development

Summary

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We describe a method for inducing neutrophilic pulmonary inflammation by challenge to aerosolized lipopolysaccharide by nebulization, to model acute lung injury. In addition, basic surgical techniques for lung isolation, tracheal intubation and bronchoalveolar lavage are also described.

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Cite this Article

Roos, A. B., Berg, T., Ahlgren, K. M., Grunewald, J., Nord, M. A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide. J. Vis. Exp. (94), e51470, doi:10.3791/51470 (2014).

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