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Kombinerad DNA-RNA Fluorescent
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Kombinerad DNA-RNA Fluorescent

Article DOI: 10.3791/51628 • 15:54 min • June 14th, 2014
June 14th, 2014 •
1, 1
1Department of Reproduction and Development, Erasmus MC - University Medical Center

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Fluorescent in situ-hybridisering (FISH) tillåter detektion av nukleinsyror i deras nativa omgivning inom celler. Vi beskriver här ett protokoll för det kombinerade, samtidig upptäckt av RNA och DNA med hjälp av FISH, som kan användas för att studera X-kromosom inaktivering i mus embryonala stamceller.

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Biokemi Lysrör kombinerad DNA-RNA FISK ES-cell cytogenetik enda cell analys X-kromosom inaktivering (XCI), Bakteriell artificiell kromosom (BAC) DNA-sond,
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