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Chapters
- 00:05Title
- 02:57Induction of Differentiation in Female Embryonic Stem Cells to Induce X Chromosome Inactivation
- 04:55Fixation of Differentiated ES Cells for Subsequent DNA-RNA FISH Analysis
- 05:33Labeling of DNA Probes for FISH Experiments
- 08:02Permeabilization, Pre-treatment and Hybridization of Fixed Cells for Combined DNA-RNA FISH
- 11:04Post-hybridization Washes and Antibody Mediated Detection of Probe
- 13:21Results: X Chromosome Inactivation in Differentiating Female ES Cells is Visualized by Combined DNA-RNA FISH
- 14:57Conclusion
Ibridazione in situ fluorescente (FISH) permette la rilevazione di acidi nucleici nel loro ambiente nativo all'interno delle cellule. Noi descriviamo qui un protocollo per la, rilevazione simultanea combinata di RNA e DNA mediante FISH, che può essere usato per studiare inattivazione del cromosoma X in cellule staminali embrionali di topo.
