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Journal / Biology / DNA-RNA combinato fluorescente
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DNA-RNA combinato fluorescente
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DNA-RNA combinato fluorescente

Article DOI: 10.3791/51628 • 15:54 min • June 14th, 2014
June 14th, 2014 •
1, 1
1Department of Reproduction and Development, Erasmus MC - University Medical Center

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Ibridazione in situ fluorescente (FISH) permette la rilevazione di acidi nucleici nel loro ambiente nativo all'interno delle cellule. Noi descriviamo qui un protocollo per la, rilevazione simultanea combinata di RNA e DNA mediante FISH, che può essere usato per studiare inattivazione del cromosoma X in cellule staminali embrionali di topo.

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Biochimica fluorescente DNA-RNA combinato FISH cellule ES citogenetica analisi di singole cellule inattivazione del cromosoma X (XCI), Batterica cromosoma artificiale (BAC) DNA-sonda,
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