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Journal
/
Immunology and Infection
/
的IFN-γ酶联免疫斑点检测,以评估水痘 - 带状疱疹病毒特异性细胞免疫继脐血移植的发展
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
Please note that all translations are automatically generated.
Click here for the English version.
的IFN-γ酶联免疫斑点检测,以评估水痘 - 带状疱疹病毒特异性细胞免疫继脐血移植的发展
DOI:
10.3791/51643-v
•
08:04 min
•
July 09, 2014
•
Insaf Salem Fourati
,
Anne-Julie Grenier
,
Élyse Jolette
,
Natacha Merindol
,
Philippe Ovetchkine
3
,
Hugo Soudeyns
3
1
Unité d'Immunopathologie Virale, Centre de Recherche du CHU Sainte-Justine, Department of Microbiology, Infectiology & Immunology, Faculty of Medicine
,
Université de Montréal
,
2
Infectious Diseases Service, CHU Sainte-Justine, Faculty of Medicine
,
Université de Montréal
,
3
Department of Paediatrics
,
Université de Montréal
Chapters
00:05
Title
01:27
Coating and Blocking the Plates
02:46
Cell Plating and Stimulation
04:40
Spot Development and Detection
05:54
Results: Representative VZV ELISpot Data
07:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
新世代的功能测定法如γ干扰素(IFN-γ)的酶联免疫斑点法,其检测细胞因子的产生在单细胞水平,并提供T细胞应答的定量和定性特征,可用于评估细胞介导的针对水痘 - 带状疱疹免疫应答病毒(VZV)。
Tags
IFN-γ ELISpot Assay
Varicella-zoster Virus
Cell-mediated Immunity
Umbilical Cord Blood Transplantation
VZV Prophylaxis
T Cell Reconstitution
VZV-specific T Cell Response
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