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Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces

JoVE Journal
Chemistry

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JoVE Journal Chemistry

Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces

Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces

DOI:

10.3791/51723-v

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08:50 min

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September 26, 2014

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Baltasar Lucendo-Villarin, Kate Cameron, Dagmara Szkolnicka, Paul Travers, Ferdous Khan, Jeffrey G. Walton, John Iredale, Mark Bradley, David C. Hay

¹MRC Centre for Regenerative Medicine,University of Edinburgh, ²School of Chemistry,University of Edinburgh, ³MRC Centre for Inflammation Research,University of Edinburgh

Chapters

00:05Title
01:37Synthesis of PHNGAD (Poly[1,6-hexanodiol/neopentyl glycol/di(ethylene glycol)-alt-adipic acid]diol
02:53Synthesis of PU134
04:18Preparation of PU134 Solutions and Coating of Glass Coverslips
05:22Irradiation of Coverslip and Cell Culture and Differentiation
06:09Results: Characterization of Polymer Coated Surfaces for Biological Applications
08:17Conclusion

Summary

Automatic Translation

This article will focus on developing polymer coated surfaces for long-term, stable culture of stem cell derived human hepatocytes.

Tags

Hepatocyte Primary Hepatocyte Stem Cell-derived Hepatocyte Cell Phenotype Biological Matrix Synthetic Polymer Coating Polyurethane Cell Culture Long-term Culture Phenotype Stability High-throughput Screening Cellular Therapy

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