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在肾小管上皮细胞胞浆钙的测量流式细胞仪
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JoVE Journal Biology
Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
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在肾小管上皮细胞胞浆钙的测量流式细胞仪

DOI:
10.3791/51857-v

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10:24 min

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October 28, 2014

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,
1Institute for Physiology, Pathophysiology, & Toxicology, Centre for Biomedical Research and Training (ZBAF),University of Witten/Herdecke, 2Institute for Immunology & Experimental Oncology, Centre for Biomedical Research and Training (ZBAF),University of Witten/Herdecke

Chapters

  • 00:05Title
  • 01:23Cell Culture and Calcium Dye Loading
  • 02:36Preparation of Cells for Flow Cytometry
  • 03:28Flow Cytometer Setup and Flow Cytometry Measurements
  • 05:33Data Analysis
  • 07:57Results: Changes in Cytosolic Calcium Concentration are Measured by Flow Cytometry
  • 09:48Conclusion

Summary

Automatic Translation

Automatic Translation

钙是参与许多生理学和病理生理学的信号通路。活细胞成像，需要专门的设备和非常耗时。使用流式细胞仪来确定在纳入悬浮贴壁的上皮细胞内钙的相对变化快速，简单的方法进行了优化。

Tags

Cytosolic CalciumFlow CytometryRenal Epithelial CellsCalcium MeasurementCalcium SignalingCalcium DyesProbenecidThapsigarginTunicamycinIonomycin

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