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在肾小管上皮细胞胞浆钙的测量流式细胞仪
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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
在肾小管上皮细胞胞浆钙的测量流式细胞仪
DOI:
10.3791/51857-v
•
10:24 min
•
October 28, 2014
•
Wing-Kee Lee
,
Thomas Dittmar
1
Institute for Physiology, Pathophysiology, & Toxicology, Centre for Biomedical Research and Training (ZBAF)
,
University of Witten/Herdecke
,
2
Institute for Immunology & Experimental Oncology, Centre for Biomedical Research and Training (ZBAF)
,
University of Witten/Herdecke
Chapters
00:05
Title
01:23
Cell Culture and Calcium Dye Loading
02:36
Preparation of Cells for Flow Cytometry
03:28
Flow Cytometer Setup and Flow Cytometry Measurements
05:33
Data Analysis
07:57
Results: Changes in Cytosolic Calcium Concentration are Measured by Flow Cytometry
09:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
钙是参与许多生理学和病理生理学的信号通路。活细胞成像,需要专门的设备和非常耗时。使用流式细胞仪来确定在纳入悬浮贴壁的上皮细胞内钙的相对变化快速,简单的方法进行了优化。
Tags
Cytosolic Calcium
Flow Cytometry
Renal Epithelial Cells
Calcium Measurement
Calcium Signaling
Calcium Dyes
Probenecid
Thapsigargin
Tunicamycin
Ionomycin
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