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小鼠胎肝文化系统解剖靶基因功能的终端红细胞生成的早期和晚期
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
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小鼠胎肝文化系统解剖靶基因功能的终端红细胞生成的早期和晚期

DOI:
10.3791/51894-v

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06:40 min

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September 09, 2014

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, , ,
1Department of Pathology,Northwestern University

Chapters

  • 00:05Title
  • 01:21Purification of Fetal Liver Erythroblasts
  • 02:32Purification of TER119 Negative Erythroblasts
  • 03:50Transduced TER119 Negative Mouse Fetal Liver Erythroblast Culture
  • 04:57Results: Representative Flow Cytometric Analyses of Differentiated and Enucleated Transduced Erythroblasts
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一个在体外小鼠胎肝红细胞培养系统剖析终端红细胞生成的早期和晚期阶段。这个系统有利于在不同的发育阶段特异性基因的功能分析。

Tags

Mouse Fetal LiverErythropoiesisErythroid Burst Forming Units (BFU-E)Erythroid Colony Forming Units (CFU-E)Terminal ErythroblastsTER119Erythroid Progenitor CellsGene Function AnalysisEarly And Late Stages Of Terminal ErythropoiesisCDNA OverexpressionShRNA KnockdownErythropoietin (Epo)

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