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人类骨髓间充质干细胞的分离及其培养的多​​孔骨基质
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
DOI:

09:00 min

February 09, 2015

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Chapters

  • 00:05Title
  • 01:15Isolation of Human Mesenchymal Stem Cells
  • 03:20Expansion of Mesenchymal Stem Cells
  • 04:37Preparation of Porous Bone Matrix Disk and Culture of Human Mesenchymal Stem Cells
  • 05:54Cell Proliferation Assay and Measuring Colony Forming Units
  • 06:56Results: Characterization of Isolated Human Mesenchymal Stem Cells
  • 08:30Conclusion

Summary

Automatic Translation

Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs the bovine bone matrix Nukbone (NKB) as a scaffold.

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