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Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging
This content is Free Access.
Chapters
- 00:05Title
- 00:54Collagen-based Scaffold Preparation
- 02:40Bioengineered Scaffold Implantation and Explant
- 04:43Bioengineered Scaffold 2-Photon Microscopy Imaging
- 07:44Results: Representative Scaffold Explant Analyses
- 09:20Conclusion
A method to create and live-image different humanized bone-marrow niches in mice is presented. Based on the supportive niche created by human mesenchymal cells, the addition of human endothelial cells induces the formation of human vessels, while the addition of rhBMP-2 induces the formation of human-mouse chimeric mature bone tissue.
