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Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems
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Chapters
- 00:05Title
- 01:30Preparation of Cells and Reagents
- 03:25Dilution and Infection
- 05:34Fixing and Staining cells
- 06:34Determining Viral Titers
- 07:02Results: Determination of Viral Titer via Plaque Assay
- 08:53Conclusion
Plaque assays are the gold standard for viral quantification, utilizing entrapping overlays on host cellular monolayers to determine viral titers. While various semisolid overlays have traditionally been used, here we demonstrate plaque techniques comparing semisolid overlays to a novel liquid microcrystalline cellulose among several families of viruses.
