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JoVE Journal
Immunology and Infection

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Viral Concentration Determination Through Plaque Assays

Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

Article DOI: 10.3791/52065
November 4th, 2014


Summary November 4th, 2014

Plaque assays are the gold standard for viral quantification, utilizing entrapping overlays on host cellular monolayers to determine viral titers. While various semisolid overlays have traditionally been used, here we demonstrate plaque techniques comparing semisolid overlays to a novel liquid microcrystalline cellulose among several families of viruses.

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