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凝胶电泳迁移分析(EMSA)为RNA,蛋白质相互作用的研究:IRE / IRP示例

凝胶电泳迁移分析(EMSA)为RNA,蛋白质相互作用的研究:IRE / IRP示例

Article doi: 10.3791/52230
December 3rd, 2014

Summary December 3rd, 2014

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在这里,我们提出了一个协议分析RNA /蛋白质相互作用。电泳迁移实验(EMSA)是基于RNA的/蛋白复合物和游离RNA在天然凝胶电泳迁移差异上。通过使用放射性标记的RNA探针,RNA /蛋白质复合物可以通过放射自显影进行显现。

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