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使用
卡诺哈布迪炎埃莱甘斯
筛选组织特异性查佩龙相互作用
JoVE Journal
Biology
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JoVE Journal
Biology
Using
Caenorhabditis elegans
to Screen for Tissue-Specific Chaperone Interactions
Please note that all translations are automatically generated.
Click here for the English version.
使用
卡诺哈布迪炎埃莱甘斯
筛选组织特异性查佩龙相互作用
DOI:
10.3791/61140-v
•
06:55 min
•
June 07, 2020
•
Shiran Dror
,
Tomer D. Meidan
,
Ido Karady
,
Anat Ben-Zvi
1
Department of Life Sciences
,
Ben-Gurion University of the Negev
Chapters
00:05
Introduction
00:30
Non‐Stressful Embryo Synchronization
02:06
Common Phenotypic Assays: Animal Cultivation, Embryonic Lethality, and Paralysis
03:30
Protein Knockdown Validation
05:02
Results: Representative Tissue‐Specific Chaperone Interaction Screening
06:26
Conclusion
Summary
Automatic Translation
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Automatic Translation
为了研究伴奏和伴奏基底相互作用,我们使用RNA干扰与轻度突变或伴郎过度表达相结合,在有机体水平上监测组织特异性蛋白质功能障碍,在
卡诺哈布德炎中
执行合成相互作用屏幕。
Tags
Caenorhabditis Elegans
Embryo Synchronization
M9 Buffer
NGM Plate
Cell Scraper
Centrifugation
Synchronized Nematodes
RNAi
Embryonic Lethality
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