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染色质免疫沉淀拟南芥蛋白-DNA相互作用的鉴定<em>在体内</em
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Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
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染色质免疫沉淀拟南芥蛋白-DNA相互作用的鉴定<em>在体内</em

English

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19,927 Views

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12:36 min

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January 14, 2016

DOI:

10.3791/53422-v

DOI:
10.3791/53422-v

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12:36 min
January 14, 2016

•

19 Views
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1Centro de Biotecnología y Genómica de Plantas (CBGP),Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-Universidad Politécnica de Madrid

Summary

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染色质免疫沉淀是一 ​​个功能强大的技术的DNA结合蛋白拟南芥的体内位点的鉴定。此过程包括染色质交联和碎裂，免疫沉淀与针对感兴趣的蛋白的选择性抗体，和结合的DNA的定量PCR分析。我们描述了拟南芥植物优化的一个简单的ChIP实验。

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Chromatin ImmunoprecipitationArabidopsisProtein-DNA InteractionsCross-linkingTranscription FactorsChromatin OrganizationPlant BiologyMagnetic BeadsChIP Dilution Buffer

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Komar, D. N., Mouriz, A., Jarillo, J. A., Piñeiro, M. Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo. J. Vis. Exp. (107), e53422, doi:10.3791/53422 (2016).

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