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JoVE Journal
Biology

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染色质免疫沉淀拟南芥蛋白-DNA相互作用的鉴定
 
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染色质免疫沉淀拟南芥蛋白-DNA相互作用的鉴定

Article DOI: 10.3791/53422
January 14th, 2016

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Summary January 14th, 2016

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染色质免疫沉淀是一 ​​个功能强大的技术的DNA结合蛋白拟南芥的体内位点的鉴定。此过程包括染色质交联和碎裂,免疫沉淀与针对感兴趣的蛋白的选择性抗体,和结合的DNA的定量PCR分析。我们描述了拟南芥植物优化的一个简单的ChIP实验。

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