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Måling af mRNA henfaldskurver i<em> Saccharomyces cerevisiae</em> Brug<em> Rpb1-1</em> Stammer
JoVE Journal
Biology
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JoVE Journal Biology
Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
DOI:

12:21 min

December 13, 2014

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Chapters

  • 00:05Title
  • 01:22Growth of Yeast Cells to Log Phase
  • 02:21Heat-induced Inhibition of mRNA Synthesis and Harvest of Yeast Cells
  • 05:18RNA Gel Electrophoresis and Northern Blot Transfer
  • 07:07Northern Hybridization
  • 08:56RNA Quantitation
  • 10:03Results: Down-regulation of an Intron Containing Pre-mRNA in NMD Mutants
  • 11:59Conclusion

Summary

Automatic Translation

Steady state-niveau af specifikke mRNA'er er bestemt af hastigheden af ​​syntese og nedbrydning af mRNA. Genom-dækkende mRNA nedbrydning satser eller henfaldet specifikke mRNA'er kan måles ved at bestemme mRNA halveringstider. Denne protokol fokuserer på måling af mRNA henfald satser i Saccharomyces cerevisiae.

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