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Chapters
- 00:05Title
- 01:22Growth of Yeast Cells to Log Phase
- 02:21Heat-induced Inhibition of mRNA Synthesis and Harvest of Yeast Cells
- 05:18RNA Gel Electrophoresis and Northern Blot Transfer
- 07:07Northern Hybridization
- 08:56RNA Quantitation
- 10:03Results: Down-regulation of an Intron Containing Pre-mRNA in NMD Mutants
- 11:59Conclusion
Steady state-niveau af specifikke mRNA'er er bestemt af hastigheden af syntese og nedbrydning af mRNA. Genom-dækkende mRNA nedbrydning satser eller henfaldet specifikke mRNA'er kan måles ved at bestemme mRNA halveringstider. Denne protokol fokuserer på måling af mRNA henfald satser i Saccharomyces cerevisiae.
