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JoVE Journal Neuroscience
Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
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Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

Article DOI: 10.3791/52361-v • 15:05 min • February 5th, 2015
February 5th, 2015 •
1, 1, 1, 1
1Research Division, Cellular Molecular Biology Branch, United States Army Medical Research Institute of Chemical Defense

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A custom protocol is described to differentiate mouse ES cells into defined populations of highly pure neurons exhibiting functioning synapses and emergent network behavior. Electrophysiological analysis demonstrates the loss of synaptic transmission following exposure to botulinum neurotoxin serotypes /A-/G and tetanus neurotoxin.

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Functional Evaluation Biological Neurotoxins Networked Cultures Stem Cell-derived Central Nervous System Neurons Therapeutic Studies Mechanistic Studies Presynaptically Targeted Clostridial Neurotoxins Scalable Cell-based Model Functioning Synapses Physiological Responses Intoxication Differentiation Protocol Embryonic Stem Cell-derived Neurons Neurotypic Proteins Neuronal Morphologies Intrinsic Electrical Responses Glutamatergic Synapses & 947;-aminobutyric Acid (GABA)ergic Synapses Network Behaviors Excitatory:inhibitory Balance Botulism Tetanus Whole-cell Patch Clamp Electrophysiology Miniature Excitatory Post-synaptic Currents (mEPSCs) Tetanus Neurotoxin (TeNT) Botulinum Neurotoxin (BoNT) Serotypes /A-/G
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