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Intrathecal Delivery of Antisense Oligonucleotides in the Rat Central Nervous System
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JoVE Journal Neuroscience
Intrathecal Delivery of Antisense Oligonucleotides in the Rat Central Nervous System

Intrathecal Delivery of Antisense Oligonucleotides in the Rat Central Nervous System

DOI:
10.3791/60274-v

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07:47 min

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October 29, 2019

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, , , , , ,
1Biogen, Inc., 2Ionis Pharmaceuticals

Chapters

  • 00:04Title
  • 00:46Material and Instrument Preparation
  • 02:09Surgery Preparation
  • 03:05Surgery
  • 06:53Results: A Single Bolus Antisense Oligonucleotide (ASO) Intrathecal Injection Reduces Rat Malat1 In Vivo
  • 07:17Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a method for delivering drugs to the rat central nervous system by implanting a catheter into the lumbar intrathecal space of the spine. We focus on the delivery of antisense oligonucleotides, though this method is suitable for delivery of other therapeutic modalities as well.

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Keywords: Intrathecal DeliveryAntisense OligonucleotidesRat Central Nervous SystemPharmacokineticsPharmacodynamicsEfficacyGuide CannulaCatheter Wire AssemblyDelivery Catheter AssemblyGuide Cannula-needle AssemblySpinal Cord InjectionBuprenorphineSurgical Procedure

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