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Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers
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Biology
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JoVE Journal Biology
Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers

Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers

DOI:
10.3791/52448-v

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08:42 min

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March 17, 2015

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, ,
1Carl Hayden Bee Research Center,USDA-ARS, 2Coupeville, WA, USA, 3Ecotoxicology Division,Eurofins Agroscience Services, Inc.

Chapters

  • 00:05Title
  • 01:36Isolating Beebread from African Honey Bee and European Honey Bee Colonies
  • 02:49Feeding Bees in Cages
  • 04:01Sampling Worker Bees and Beebread and Estimating Consumption
  • 05:09Protein Analysis
  • 06:34Results: pH and Protein Analysis in Beebread
  • 08:09Conclusion

Summary

Automatic Translation

Automatic Translation

Here are methods to quantify nutrients in pollen before and after its conversion to beebread by two subspecies of honeybees. We describe techniques to measure beebread consumption and resulting protein titers in both subspecies.

Tags

BeebreadPollenProteinAmino AcidHemolymph ProteinAfricanized Honey BeesEuropean Honey BeesProtein CompositionProtein AcquisitionEnvironmental Adaptation

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