Journal
/
/
Struktur og funktion Studier i embryonale stamceller fra mus Brug rekombinase-medieret Cassette Exchange
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
DOI:

15:13 min

April 27, 2017

, , , , , , , , , ,

Chapters

  • 00:05Title
  • 01:11The RMCE Principle
  • 02:15Isolation of RMCE-compatible Knockout Mouse ES Cells
  • 05:22Isolate RMCE-compatible Knockout ES Cells
  • 07:29Generation of an RMCE-compatible Targeting Vector Using Recombination Cloning
  • 09:25RMCE-mediated ES Cell Targeting of Rescue Constructs to the R26 Locus and Differentiation of ES Cells in Embryoid Bodies
  • 12:41Results: Cystic EB Formation Using Recombinase Mediated Cassette Exchange
  • 14:27Conclusion

Summary

Automatic Translation

Proteiner indeholder ofte flere domæner, som kan øve forskellige cellulære funktioner. Gene knock-outs (KO) anser ikke denne funktionel diversitet. Her rapporterer vi en rekombinationsmedieret kassette udveksling (RMCE) -baseret struktur-funktion tilgang i KO embryonale stamceller, som giver mulighed for den molekylære dissektion af forskellige funktionelle domæner eller varianter af et protein.

Related Videos

Read Article