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Developmental Biology

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JoVE Journal Developmental Biology
Struktur og funktion Studier i embryonale stamceller fra mus Brug rekombinase-medieret Cassette Exchange
Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
Journal (Developmental Biology)
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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Journal (Developmental Biology)
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Struktur og funktion Studier i embryonale stamceller fra mus Brug rekombinase-medieret Cassette Exchange

Article DOI: 10.3791/55575 • 15:13 min
April 27th, 2017 •
1,2,3,4, 1,2, 1,2,4, 1,2,4, 1,2, 5, 6, 1,2,4, 7, 1,2,4, 1,2,3,4
1Department of Biomedical Molecular Biology, Ghent University, 2Inflammation Research Center, VIB, 3Center for Medical Genetics, Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre

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April 27th, 2017

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Proteiner indeholder ofte flere domæner, som kan øve forskellige cellulære funktioner. Gene knock-outs (KO) anser ikke denne funktionel diversitet. Her rapporterer vi en rekombinationsmedieret kassette udveksling (RMCE) -baseret struktur-funktion tilgang i KO embryonale stamceller, som giver mulighed for den molekylære dissektion af forskellige funktionelle domæner eller varianter af et protein.

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