Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Isolering og karakterisering af satellit-celler…
JoVE Journal
Developmental Biology

This content is Open Access.

JoVE Journal Developmental Biology
Isolering og karakterisering af satellit-celler fra rotte Hoved Branchiomeric Muskler
The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging
Journal (Developmental Biology)
The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging
Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart
Journal (Developmental Biology)
Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart
 
Click here for the English version

Isolering og karakterisering af satellit-celler fra rotte Hoved Branchiomeric Muskler

Article DOI: 10.3791/52802-v • 07:37 min • July 20th, 2015
July 20th, 2015 •
1, 2, 3, 1, 1, 1
1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 2Department of Biological Structure, University of Washington School of Medicine, 3Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol beskriver isoleringen af ​​satellit celler fra branchiomeric hoved muskler en 9 uger gammel rotte. Musklerne stammer fra forskellige Branchialfodder buer. Efterfølgende satellit-celler dyrkes på en plet belægning af millimeter størrelse at studere deres differentiering. Denne fremgangsmåde undgår udvidelse og passage af satellit celler.

Tags

Developmental Biology hoved muskler levator veli Palatini muskel digastric muskler kæbemuskel satellit celler isolation primærelementer ganespalte regenerativ medicin tissue engineering stamceller differentiering myofibre
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter