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正向遗传学的方法来揭开抗逆基因小鼠 - 在ES细胞高通量筛选
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Developmental Biology
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JoVE Journal
Developmental Biology
Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells
Please note that all translations are automatically generated.
Click here for the English version.
正向遗传学的方法来揭开抗逆基因小鼠 - 在ES细胞高通量筛选
DOI:
10.3791/53062-v
•
15:40 min
•
November 11, 2015
•
Michael Ludwig
,
David Kitzenberg
,
Wallace S. Chick
1
Department of Cell and Developmental Biology
,
University of Colorado Denver
Chapters
00:05
Title
00:48
Construction of a Gene-trapped Embryonic Stem (ES) Cell Library
06:11
Replication of the ES Cell Library
09:49
Stress Selection and Isolation of Resistant Clones
13:14
Results: Forward Genetic Screen for Stress Resistance Mutations
14:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
应力性的标志长寿之一,已知是基因支配。在这里,我们开发了一个不带偏见的高通量方法筛选,赋予抗逆性ES细胞以开发小鼠模型长寿研究突变。
Tags
Genetic Screen
Forward Genetics
Embryonic Stem Cells
High-throughput
Stress Resistance
Paraquat
Oxidative Stress
Mouse Models
Gene Function
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