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Air-שנדגמו ניתוח סינון עבור אנדוטוקסינים ותוכן DNA
JoVE Journal
Environment
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JoVE Journal Environment
Air-sampled Filter Analysis for Endotoxins and DNA Content
DOI:

09:16 min

March 07, 2016

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Chapters

  • 00:05Title
  • 00:34Air Filter Preparation and Sampling
  • 01:38Extraction of Endotoxin from Filter Samples
  • 02:51Limulus Amebocyte Lysate Endotoxin Test
  • 04:30DNA Extraction from Experimental and Spiked Filters
  • 06:02Quantitative PCR Analysis
  • 07:48Results: Centrifugation Speed and Filter Load Influences the Measured Endotoxin Concentrations in Samples
  • 08:35Conclusion

Summary

Automatic Translation

Two complementary analyses of atmospheric biological particles from air sampled filters are described herein: the extraction and detection of endotoxin, and of DNA.

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