Journal
/
/
基于生物发光钙指标GFP的水母体内脑功能成像方法
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin
DOI:

12:15 min

January 08, 2016

, ,

Chapters

  • 00:05Title
  • 00:57Positioning and Gluing of Drosophila
  • 02:23Drosophila Dissection
  • 03:58Imaging Setup and Sample Preparation
  • 05:47Image Recording
  • 07:39Extracting Photon Values
  • 09:51Results: In Vivo Functional Brain Imaging
  • 11:00Conclusion

Summary

Automatic Translation

在这里,我们提出了一个新颖的利用生物发光记者-imaging方法。这种方法使用的稠构建GFP-水母发光蛋白,其结合 Ca 2+并发光,省去了光激发。显著此方法允许长时间连续成像,获得脑深部的结构和高时间分辨率。

Related Videos

Read Article