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评估抗癌药物的特异性<em>体外</em
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Assessing Specificity of Anticancer Drugs
In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
评估抗癌药物的特异性<em>体外</em
DOI:
10.3791/53752-v
•
09:39 min
•
March 23, 2016
•
Lan Kluwe
2,3
1
Department of Maxillofacial Surgery
,
University Medical Center Hamburg-Eppendorf
,
2
Department of Neurology
,
University Medical Center Hamburg-Eppendorf
,
3
Laboratory for Tumor Biology and Regenerative Medicine
,
University Medical Center Hamburg-Eppendorf
Chapters
00:05
Title
01:00
Mix Culture of Both Tumor Cells and Non-tumor Cells and Drug Treatment
02:44
Preparing DNA for Digital PCR
04:51
Digital PCR
06:22
Calculating Tumor Cell Proportion
07:20
Results: Dose-Dependent Change of Tumor Cell Proportion and Responses of Tumor/Non-Tumor Cells
08:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目的是评估使用同时含有肿瘤和非肿瘤细胞混合培养抗癌药物
在体外
的特异性。
Tags
In Vitro
Anticancer Drugs
Tumor Cells
Non-tumor Cells
Specificity
Drug Discovery
Personalized Cancer Care
Cell Culture
Nilotinib
Dose-dependent
HotSHOT Method
Cell Lysis
PCR
DNA
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