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Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research
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Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

DOI:
10.3791/55704-v

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08:30 min

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June 08, 2017

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, ,
1Eaton Peabody Laboratories, Department of Otolaryngology,Massachusetts Eye and Ear, 2Department of Otolaryngology,Harvard Medical School, 3Department of Otolaryngology,Vienna General Hospital, Medical University of Vienna, 4Harvard Program in Speech and Hearing Bioscience and Technology

Chapters

  • 00:05Title
  • 00:53Macroscopic Dissection of the Temporal Bone
  • 02:06Microscopic Extraction of the Cochleae
  • 04:31Culture of Cochlear Explants
  • 05:59Treatment of Explants
  • 06:38Results: Research Applications of Cochlear Explants
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to demonstrate the preparation, culture, treatment, and immunostaining of neonatal murine cochlear explants. The technique can be utilized as an in vitro screening tool in hearing research.

Tags

Keywords: Neonatal MurineCochlear ExplantIn VitroHearing ResearchHair CellsSynapsesNeuritesNeuronsEx VivoTherapeutic CompoundsScreening ToolDissectionOrgan Of CortiSpiral Ligament

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