In Vitro Model of Coronary Angiogenesis

Our protocol is significant because it allows researchers to investigate the molecular mechanism of coronary angiogenesis outside of an organism. The main advantage of this technique is that it accurately models the process of coronary angiogenesis inside an organism, facilitating the study of the mechanisms of coronary angiogenesis. Begin by spraying a pregnant mouse carrying embryonic day 11.5 embryos with 70%ethanol.

Lifting the skin over the belly with forceps, use scissors to make an abdominal skin and peritoneal incision laterally and anteriorly up to the diaphragm to expose the uterine horn. Grasping the uterus, cut the tissue free and pull out the uterine horn. Place the string of embryos into ice cold sterile PBS under a stereo microscope, and peel off the uterine muscle, yolk sac, and amnion cover from each embryo.