At the subcellular level, signaling events are dynamically modulated by developmental and environmental cues. Here we describe a protocol that employs the tobacco transient expression system to monitor dynamic protein-protein interaction and to disclose spatial organization of signal transduction in plant cells.
Zhang, Y., Dong, J. Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System. J. Vis. Exp. (109), e53790, doi:10.3791/53790 (2016).