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Production of Human Norovirus Protruding Domains in E. coli for X-ray Crystallography
 

Production of Human Norovirus Protruding Domains in E. coli for X-ray Crystallography

Article DOI: 10.3791/53845
April 19th, 2016

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Summary April 19th, 2016

Here, we describe a method to express and purify high quality norovirus protruding (P) domains in E. coli for use in X-ray crystallography studies. This method can be applied to other calicivirus P domains, as well as non-structural proteins, i.e., viral protein genome-linked (VPg), protease, and RNA dependent RNA polymerase (RdRp).

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