30,358 Views
•
09:01 min
•
March 16, 2011
DOI:
Summary
Automatically generated
Here we demonstrate a simple protocol to create a random mutant library for a given target sequence. We show how this method, which is performed in vivo in Escherichia coli, can be coupled with functional selections to evolve new enzymatic activities.
Related Videos
06:03
Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
Related Videos
14164 Views
07:18
Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
Related Videos
10596 Views
13:30
A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
Related Videos
17844 Views
07:04
Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
Related Videos
19757 Views
09:16
In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
Related Videos
7182 Views
10:27
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
Related Videos
8813 Views
11:25
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Related Videos
6523 Views
11:08
A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
Related Videos
6803 Views
12:48
The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Related Videos
11832 Views
10:50
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
Related Videos
10826 Views
Read Article
Cite this Article
Troll, C., Alexander, D., Allen, J., Marquette, J., Camps, M. Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli. J. Vis. Exp. (49), e2505, doi:10.3791/2505 (2011).
Copy