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鼠冠状动脉血管平滑肌细胞的分离

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Biology

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JoVE Journal Biology

Isolation of Murine Coronary Vascular Smooth Muscle Cells

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鼠冠状动脉血管平滑肌细胞的分离

DOI:

10.3791/53983-v

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08:24 min

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May 30, 2016

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Kathryn E. Husarek², Xiaojin Zhang, Patricia E. McCallinhart, Pamela A. Lucchesi³, Aaron J. Trask³

¹School of Biomedical Science,The Ohio State University College of Medicine, ²Center for Cardiovascular and Pulmonary Research,The Research Institute at Nationwide Children’s Hospital, ³Department of Pediatrics,The Ohio State University College of Medicine

Chapters

00:05Title
00:43Heart Isolation and Cannulation
04:00Digestion and Vascular Smooth Muscle Cell (VSMC) Isolation
06:08VSMC Culture
07:10Results: Representative Cultured Coronary VSMC
07:58Conclusion

Summary

Automatic Translation

此协议的目的是演示从冠脉循环鼠主要血管平滑肌细胞的分离和培养技术（血管平滑肌细胞）。一旦血管平滑肌细胞已被分离，它们可用于许多标准培养技术。

Tags

Murine Coronary Vascular Smooth Muscle Cells Primary Coronary Vascular Smooth Muscle Cells Coronary Circulation Cardiovascular Field Disease Mechanisms Cell Culture Vascular Smooth Muscle Cell Behavior Isolation Perfusion Cannulation Heparin Heart Excision Aorta Cell Culture Assays

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