Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

The overall goal of this procedure is to observe anti-cancer therapeutic response in single cells in culture. This method can answer key questions in the cancer research field, including heterogeneous therapeutic response and cell fate. To begin, under a certified sterile laminar flow hood, prepare HT1080 cells in culture-certified dishes according to the text protocol and incubate the cells in a humidified cell culture incubator.

Two days prior to imaging, plate 50, 000 HT1080 FuGENE cells per well into the desired number of wells of a 12-well, no. 1.5 glass bottomed dish. Adjust the number of cells plated depending on their growth rate and the dish used in order to achieve approximately 60%confluence for the start of the experiment.