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JoVE Journal Genetics
siRNA Transfeksjon og EMSA Analyser på nylig isolerte humane villøse Cytotrophoblasts
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siRNA Transfeksjon og EMSA Analyser på nylig isolerte humane villøse Cytotrophoblasts

Article DOI: 10.3791/53995-v • 09:57 min • September 20th, 2016
September 20th, 2016 •
1, 2, 3, 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

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Denne protokollen beskriver en fremgangsmåte for effektiv transfeksjon av siRNA i nylig isolerte humane villous cytotrophoblasts ved hjelp microporation og identifisering av DNA-proteinkomplekser i disse cellene. Transfekterte celler kan overvåkes ved hjelp av Western blot-analyser og EMSA i løpet av 4 dagers kultur tid.

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Genetikk Molecular Biology Human villous cytotrophoblasts Syncytin-2 siRNA Microporation Lipid-baserte transfeksjon Elektro Mobility Skift Assay
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