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Listeria monocytogenes Infection of the Brain
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Immunology and Infection
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JoVE Journal Immunology and Infection
Listeria monocytogenes Infection of the Brain

Listeria monocytogenes Infection of the Brain

DOI:
10.3791/58723-v

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05:02 min

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October 02, 2018

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1Department of Microbiology and Immunobiology,Harvard Medical School

Chapters

  • 00:04Title
  • 00:40L. monocytogenes-infected Mouse Dissection and Cardiac Perfusion
  • 01:51Organ Harvest and Bacterial Burden Determination
  • 04:00Results: Representative Bacterial Burdens in Infected Mouse Organs
  • 04:22Conclusion

Summary

Automatic Translation

Automatic Translation

During infection, Listeria monocytogenes is capable of crossing the blood-brain barrier to colonize the brain. In this protocol, we demonstrate how to assess bacterial colonization of organs following infection of mice. A procedure to perform whole organ perfusion for specific determination of bacterial numbers in the brain parenchyma is provided.

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Keywords: Listeria MonocytogenesBrain InfectionBlood-brain BarrierBacterial PathogenesisPerfusionBrain Dissection

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