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相关光学和电子显微镜,研究与β淀粉样蛋白斑块的小神经胶质互动
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques
DOI:

10:52 min

June 01, 2016

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Chapters

  • 00:05Title
  • 00:57Methoxy-X04 Solution Preparation and Injection
  • 01:52Brain Sectioning Using a Vibratome
  • 03:21Screening for the Presence of Methoxy-X04-stained Plaques
  • 05:40Tissue Processing for Electron Microscopy
  • 09:01Results: Visualization of Aβ Plaques in 21-month-old APP-PS1 Mice Using Light Microscopy Following Systemic Injection of the Fluorescent Dye Methoxy-X04
  • 10:01Conclusion

Summary

Automatic Translation

本文介绍了使用甲氧基X04,它穿过血 - 脑屏障,并选择性地结合β褶的致密核心斑块Aβ发现张在阿尔茨海默氏症小鼠模型可视化淀粉样蛋白斑块Aβ的协议。它允许的前免疫染色和处理电子显微镜含斑块组织切片预检。

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