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Biology
Biology
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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
August 13th, 2016
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Chapters
Summary
The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.
Tags
Reconstitution Basic Mitotic Spindles Spherical Emulsion Droplets Mitotic Spindle Assembly Individual Components Bottom-up Approach Geometrical Confinement Mimics Shape Of A Mitotic Cell Cellular Confinement PDMS Pre-polymer Curing Agent Vacuum Chamber Aluminum Foil Mold Glass Slides Spin Coating Harden PDMSRelated Videos
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