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Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
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JoVE Journal Biology
Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

DOI:
10.3791/60478-v

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08:31 min

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November 15, 2019

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,
1AG Oncophysiology,Max-Planck Institute of Experimental Medicine, 2Göttingen Graduate School for Neurosciences, Biophysics, and Molecular Biosciences (GGNB)

Chapters

  • 00:04Title
  • 01:30Cell Seeding and pEB3-tdTomato Expression
  • 02:37Synchronization and Live-cell Imaging
  • 03:56Analysis of Microtubule Dynamics
  • 06:29Results: Analysis of Microtubule Dynamics in HeLa Cells Synchronized in Prometaphase
  • 07:50Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a robust and detailed method of microtubule dynamics analysis in cells synchronized in prometaphase using live-cell spinning disk confocal microscopy and MATLAB-based image processing.

Tags

Keywords: Microtubule DynamicsSpinning Disk MicroscopyMonopolar Mitotic SpindlesFluorescent ProteinPhototoxicityEB3TrypsinizationHeLa CellsTransfectionDimethylenastron (DME)

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