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JoVE Journal
Biology

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Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
 

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Article DOI: 10.3791/60478-v
November 15th, 2019

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Summary November 15th, 2019

Here we present a robust and detailed method of microtubule dynamics analysis in cells synchronized in prometaphase using live-cell spinning disk confocal microscopy and MATLAB-based image processing.

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