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Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
 

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

Article doi: 10.3791/52427
March 9th, 2015

Summary March 9th, 2015

Described here is a rapid and effective procedure for functional reconstitution of purified wild-type and mutant CFTR protein that preserves activity for this chloride channel, which is defective in Cystic Fibrosis. Iodide efflux from reconstituted proteoliposomes mediated by CFTR allows studies of channel activity and the effects of small molecules.

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