Journal
/
Immunology and Infection
/
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions…
JoVE Journal
Immunology and Infection
Immunology and Infection
A subscription to JoVE is required to view this content.
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
October 30th, 2016
•
1Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology, Johann Wolfgang Goethe-University, 2LOEWE Center for Cell and Gene Therapy, Johann Wolfgang Goethe-University, 3Institute for Cancer Research, Department of Cancer Immunology, Oslo University Hospital, Radiumhospital, 4The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine, University of Oslo
* These authors contributed equally
Chapters
Summary
A simple and reliable method is described here to analyze a set of NK cell functions such as degranulation, cytokine and chemokine production within different NK cell subsets.
Tags
Flow Cytometry-based Assay NK Cell Functions Monitoring Viral Infections Malignant Diseases Scientific Research Clinical Evaluations Pediatrics Immunodeficient Patients NK Cell Subsets NK Cell Isolation Cocktail Solution EDTA Peripheral Whole Blood Tube Rotator Magnetic Separator Sterile Conditions Complete MediumRelated Videos
PLAYLIST
Cell Therapy
-
Research • Cancer ResearchA Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
-
Education • ImmunologyAdoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS
-
Research • Immunology and InfectionA Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
-
Research • BioengineeringConstruction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
-
Research • GeneticsAssessing Stem Cell DNA Integrity for Cardiac Cell Therapy
-
Research • Bioengineering3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
Protocols for Methods Used in Cell Therapy
-
Research • BiologyIsolation of Immune Cells from Primary Tumors
-
Research • Immunology and InfectionDirected Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes
-
Research • Cancer ResearchIsolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment
-
Research • EngineeringEnrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
-
Research • Immunology and InfectionHigh-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
-
Research • Developmental BiologyChondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
Immune Cells in Therapy
-
Research • Cancer ResearchManufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy
-
Research • Immunology and InfectionExpansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
-
Research • Immunology and InfectionFlow Cytometry-based Assay for the Monitoring of NK Cell Functions
-
Research • Immunology and InfectionGeneration of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
-
Research • Immunology and InfectionClinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood
CAR-T Therapies (Autologous)
-
Research • BioengineeringTransduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
-
Research • Immunology and InfectionGeneration of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care
-
Research • Developmental BiologyGeneration of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes
-
Research • Cancer ResearchA Spheroid Killing Assay by CAR T Cells
Allogenic CAR-T Therapy
-
Research • BioengineeringTransplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
-
Research • BehaviorUsing the Activity-based Anorexia Rodent Model to Study the Neurobiological Basis of Anorexia Nervosa
-
Research • MedicineEstablishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment
-
Research • Immunology and InfectionStereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts
-
Research • Immunology and InfectionIn Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients