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Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

DOI:
10.3791/64344-v

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12:46 min

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August 17, 2022

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, ,
1Division of Cardiology, Department of Medicine,The Johns Hopkins University School of Medicine

Chapters

  • 00:04Introduction
  • 01:05Intravascular B-Cell Staining and Heart Collection
  • 02:58Heart Digestion and Cell Staining
  • 05:35Flow Cytometry
  • 11:31Results: Representative Plots Reporting the Average Number of B-Cells Per Milligram of Myocardial Tissue
  • 12:12Conclusion

Summary

Automatic Translation

Automatic Translation

Here we report a protocol for the quantification and differentiation of myocardial B-lymphocytes based on their location in the intravascular or endothelial space using flow cytometry.

Tags

Keywords: Flow CytometryMyocardial B-cellsPerfusionDigestionIsolationAntibody PanelImmune Cell TypesHeartLungLiverB220 AntibodyEuthanasiaHBSSMinced HeartEnzymesDNase ICollagenase IIHyaluronidase

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