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Journal
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Immunology and Infection
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流式细胞仪为基础的检测方法的NK细胞功能的监控
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
Please note that all translations are automatically generated.
Click here for the English version.
流式细胞仪为基础的检测方法的NK细胞功能的监控
DOI:
10.3791/54615-v
•
08:17 min
•
October 30, 2016
•
Sara Tognarelli*
1,2
,
Benedikt Jacobs*
3,4
,
Nina Staiger
2
,
Evelyn Ullrich
2
1
Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology
,
Johann Wolfgang Goethe-University
,
2
LOEWE Center for Cell and Gene Therapy
,
Johann Wolfgang Goethe-University
,
3
Institute for Cancer Research, Department of Cancer Immunology
,
Oslo University Hospital, Radiumhospital
,
4
The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine
,
University of Oslo
Chapters
00:05
Title
01:01
NK Cell Isolation
02:20
NK Cell Stimulation
03:52
Surface and Intracellular Staining
06:04
Results: Representative Healthy Donor NK Cell Stimulation Analysis
07:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
一种简单和可靠的方法是此处描述的分析的一组NK细胞的功能,如不同的NK细胞亚群内脱粒,细胞因子和趋化因子生产。
Tags
Flow Cytometry
NK Cell Functions
Natural Killer Cells
Viral Infections
Malignant Diseases
Pediatrics
Immunodeficient Patients
NK Cell Isolation
EDTA Peripheral Whole Blood
NK Cell Stimulation
K562 Tumor Cells
IL-2
IL-15
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