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细胞外基质蛋白纤维的布里渊谱的制备
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
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细胞外基质蛋白纤维的布里渊谱的制备

DOI:
10.3791/54648-v

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07:19 min

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September 15, 2016

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, , , , , ,
1School of Physics and Astronomy,University of Exeter, 2Department of Physics and Geology,University of Perugia, 3Istituto Officina dei Materiali del CNR,Unità di Perugia

Chapters

  • 00:05Title
  • 00:46Rat Tail Collagen Fiber Extraction
  • 02:16Bovine Nuchal Ligament Elastin Fiber Extraction
  • 03:44Reflective Substrate Fiber Mounting, Brillouin Experiment Setup and Fiber Spectra Acquisition
  • 04:55Results: Representative Micromechanics of Collagen and Elastin Fibers
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.

Tags

Extracellular MatrixCollagenElastinBrillouin SpectroscopyTendonChondroitinase ABCStreptomyces HyaluronidaseTrypsinBovine Nuchal LigamentSodium HydroxideWater Bath

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