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Neuroscience
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第一次混合胶质细胞培养的成年小鼠脊髓组织准备
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Neuroscience
Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue
Please note that all translations are automatically generated.
Click here for the English version.
第一次混合胶质细胞培养的成年小鼠脊髓组织准备
DOI:
10.3791/54801-v
•
07:13 min
•
November 19, 2016
•
Jennifer T. Malon
,
Ling Cao
1
Department of Biomedical Sciences, College of Osteopathic Medicine
,
University of New England
Chapters
00:05
Title
00:55
Preparation of the Single Cell Suspension
03:12
Removal of Myelin from the Single Cell Suspension
05:03
Results: Mixed Glial Cultures and Response to Stimuli
06:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
神经性疼痛的发展涉及脊髓神经胶质细胞的病理变化。从成年脊髓组织的,设计用于
在体外
研究这些细胞中的胶质可靠的培养体系缺乏。因此,我们在这里展示了如何建立从成年小鼠的脊髓组织的主要胶质细胞混合培养。
Tags
Primary Mixed Glial Cultures
Adult Mouse Spinal Cord
In Vitro Studies
Neuropathic Pain
Multiple Sclerosis
HBSS
Papain DNAse
Enzyme Digestion
Single Cell Suspension
Albumin Ovomucoid Inhibitor
DNAse
Centrifugation
Myelin Removal
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