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Biochemistry
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检查蛋白酶体大会与重组古菌蛋白酶体和非变性PAGE:该案例相结合的办法
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Biochemistry
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JoVE Journal
Biochemistry
Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
Please note that all translations are automatically generated.
Click here for the English version.
检查蛋白酶体大会与重组古菌蛋白酶体和非变性PAGE:该案例相结合的办法
DOI:
10.3791/54860-v
•
09:57 min
•
December 17, 2016
•
Dilrajkaur Panfair
,
Andrew R. Kusmierczyk
1
Department of Biology
,
Indiana University-Purdue University, Indianapolis (IUPUI)
Chapters
00:05
Title
00:43
Bacterial Lysis and Lysate Mixing
02:31
Protein Purification via Immobilized Cobalt Affinity Resin (ICAR)
05:04
Nondenaturing Polyacrylamide Gel Electrophoresis (PAGE)
08:09
Results: A Combined Approach for Assaying Proteasome Assembly
09:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议使用两个亚基共表达和postlysis亚基重组蛋白酶组件的更彻底的检查混合。
Tags
Proteasome Assembly
Recombinant Archaeal Proteasomes
Nondenaturing PAGE
Assembly Intermediates
Eukaryotic Proteasome
Archaeal 20S Proteasomes
Coexpression
Lysate Mixing
Protein Complex Assembly
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