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High-resolution respirometrietest aan mitochondriale functie beoordelen in gepermeabiliseerde en intacte cellen
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High-resolution respirometrietest aan mitochondriale functie beoordelen in gepermeabiliseerde en intacte cellen

Article DOI: 10.3791/54985-v • 08:33 min • February 8th, 2017
February 8th, 2017 •
1, 1
1Department of Intensive Care Medicine, Inselspital, Bern University Hospital, University of Bern

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Hoge-resolutie respirometrie wordt gebruikt om mitochondriale zuurstofconsumptie bepalen. Dit is een eenvoudige techniek om mitochondriale ademhalingsketen complexen (I-IV) ademhalingssnelheid, maximale mitochondriale elektronentransport systeemcapaciteit en buitenste mitochondriale membraan integriteit te bepalen.

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Cellular Biology mitochondria oxidatieve fosforylering zuurstofverbruik hoge-resolutie respirometrie cytochroom C digitonine
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