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10:06 min
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October 19, 2013
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Summary
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De zebravis, een kleine tropische vissen, is uitgegroeid tot een populair model voor het bestuderen van gen-functie tijdens gewervelde ontwikkeling en ziekte. De ruimtelijke en temporele expressie van de doelwitgenen worden bepaald<em> In situ</em> Hybridisatie. Onze verbeterde protocol maakt de detectie van lage overvloedige transcripten met lage niet-specifieke achtergrondsignaal.
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Cite this Article
Chitramuthu, B. P., Bennett, H. P. J. High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function. J. Vis. Exp. (80), e50644, doi:10.3791/50644 (2013).
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