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A蛋白制备方法在蛋白质与核余子交互使用LC-MS / MS分析的高通量鉴定
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
DOI:

05:43 min

January 24, 2017

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Chapters

  • 00:05Title
  • 00:45Protein Extraction
  • 02:01Immunoprecipitation
  • 03:47Alkylation and Trypsinization
  • 04:46Results: Purification of ARIP4 Complexes
  • 05:13Conclusion

Summary

Automatic Translation

我们已经建立了使用LC-MS / MS系统共同调节相互作用蛋白的纯化的方法。

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