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Biochemistry
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A蛋白制备方法在蛋白质与核余子交互使用LC-MS / MS分析的高通量鉴定
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Biochemistry
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Please note that all translations are automatically generated.
Click here for the English version.
A蛋白制备方法在蛋白质与核余子交互使用LC-MS / MS分析的高通量鉴定
DOI:
10.3791/55077-v
•
05:43 min
•
January 24, 2017
•
Megumi Tsuchiya*
1
,
M. Rezaul Karim*
2
,
Taro Matsumoto
,
Hidesato Ogawa
,
Hiroaki Taniguchi
4,5
1
Graduate School of Frontier Biosciences
,
Osaka University
,
2
Department of Biotechnology and Genetic Engineering
,
Jahangirnagar University
,
3
Division of Cell Regeneration and Transplantation
,
School of Medicine, Nihon University
,
4
Institute of Genetics and Animal Breeding of the Polish Academy of Sciences
,
5
Graduate School of Life and Medical Sciences
,
Doshisha University
Chapters
00:05
Title
00:45
Protein Extraction
02:01
Immunoprecipitation
03:47
Alkylation and Trypsinization
04:46
Results: Purification of ARIP4 Complexes
05:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们已经建立了使用LC-MS / MS系统共同调节相互作用蛋白的纯化的方法。
Tags
Protein Preparation
High-throughput
Protein Interactions
Nuclear Cofactor
LC-MS/MS Analysis
Transcriptional Mechanisms
Gene Regulation
Flag-tagged ARIP4
HEK 293 Cells
Cell Harvesting
Cell Lysis
Immunoprecipitation
Protein Elution
Mass Spectrometry
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