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Journal / Genetics / Genome Redigering og Regissert Differensiering av …
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Genetics

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JoVE Journal Genetics
Genome Redigering og Regissert Differensiering av hPSCs for Henting Lineage Determinanter i Human bukspyttkjertelen Development
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Genome Redigering og Regissert Differensiering av hPSCs for Henting Lineage Determinanter i Human bukspyttkjertelen Development

Article DOI: 10.3791/55267-v • 09:37 min • March 5th, 2017
March 5th, 2017 •
*1, *1, *1, 1
1Developmental Biology Program, Sloan Kettering Institute
* These authors contributed equally

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Protokoller for å generere hPSC mutant linjer ved hjelp av iCRISPR plattform og å skille hPSCs til glukose-responsive e-lignende celler er beskrevet. Kombinere genom redigering teknologi med hPSC rettet differensiering gir en kraftig plattform for systematisk analyse av rollen avstamning determinants i menneskelig utvikling og progresjon av sykdommen.

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Genetikk CRISPR / Cas9 iCas9 genom redigering AAVS1 menneskelige pluripotente stamceller bukspyttkjertel differensiering glukose-responsive e-lignende celler diabetes
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