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液相色谱离子迁移 QTOF 质谱测定蛋白质生物中宿主细胞蛋白的 HS-MRM 法
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal
Chemistry
An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
Please note that all translations are automatically generated.
Click here for the English version.
液相色谱离子迁移 QTOF 质谱测定蛋白质生物中宿主细胞蛋白的 HS-MRM 法
DOI:
10.3791/55325-v
•
11:09 min
•
April 17, 2018
•
Catalin Doneanu*
1
,
Jing Fang*
1
,
Yun Alelyunas*
1
,
Ying Qing Yu
,
Mark Wrona
,
Weibin Chen
1
Waters Corporation
Chapters
00:04
Title
00:44
Setup of the LC/HDMS
E
(High Definition MS
E
) Data Acquisition Method
03:59
Setup of the Tof-MRM Method for Collision Energy Optimization and the Final HS-MRM Acquisition Method for Peptide Quantification
07:42
Creation of a Processing Method for the Analysis of the HS-MRM Dataset
09:57
Results: An HS-MRM Assay for Quantification of Host-cell Protein Peptides in a Monoclonal Antibody Digest
10:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们描述了一种色谱检测, 再加上多肽前体离子迁移分离的高分辨率 (~ 3万) MS-肽片段的检测, 用于定量测定单克隆抗体中的尖肽标准消化。
Tags
HS-MRM Assay
Host-cell Proteins
Protein Biopharmaceuticals
Liquid Chromatography
Ion Mobility
QTOF Mass Spectrometry
Peptide Quantification
Protein Characterization
Quantitative Proteomics
Low-abundant Proteins
Peptide Separation
Gradient Elution
Ion Mobility Separation
High-resolution Mass Spectrometry
High-definition MSE
Collision Energy
Lock Mass Correction
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